WebT4 RNA Ligase 2 (NEB), enzyme and buffers HEPES (Fisher), as a 0.5 M stock, adjusted to pH 7.4 with potassium hydroxide, sterile filtered Potassium chloride (Fisher), as a 1.5 M stock, sterile filtered RepX helicase (e.g., 5 μM stock), prepared as described previously ( Arslan et al., 2015) Biotinylated bovine serum albumin (BSA, Sigma) WebJan 7, 2013 · T4 polynucleotide kinase-phosphatase (Pnkp) exemplifies a family of enzymes with 5'-kinase and 3'-phosphatase activities that function in nucleic acid repair. The polynucleotide 3'-phosphatase reaction is executed by the Pnkp C-terminal domain, which belongs to the DxDxT acylphosphatase superfamily. …
manual NEBNext DNA Library Prep Reagent Set for Illumina …
WebNov 9, 2010 · Enhancement of T4 DNA ligase activity by supplemental oligonucleotides. (a) Unsuccessful 4-bp duplex reactions could be salvaged by utilizing a supplementary oligonucleotide, designed to complement the first oligonucleotide-dsDNA duplex but is unphosphorylated to prevent ligation of itself. WebT4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP T4 Polynucleotide Kinase exhibits eaglercraft resource packs
T4 Polynucleotide Kinase Reaction Buffer NEB
WebT4 Polynucleotide Kinase Reaction Buffer NEB Other T4 Polynucleotide Kinase Reaction Buffer *On-line ordering is for Canadian customers only. Web pricing is … WebT4 polynucleotide kinase (NEB), enzyme and buffers T4 RNA Ligase 2 (NEB), enzyme and buffers HEPES (Fisher), as a 0.5 M stock, adjusted to pH 7.4 with potassium hydroxide, sterile filtered Potassium chloride (Fisher), as a 1.5 M stock, sterile filtered RepX helicase (e.g., 5 μM stock), prepared as described previously ( Arslan et al., 2015) WebThermo Scientific™ T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs … eaglercraft offline download