WebCypHer5E is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E can be detected with the filter sets for Cy5 TM. This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related … WebCypHer5E is a red excited fluorescent, pH sensitive cyanine dye. It is non-fluorescent at basic pH and is fluorescent at acidic pH, with a pKa of 7.3. The CypHer5E Mono NHS …
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WebThe fluorescence of the cypHer dye is almost quenched at the neutral extra-cellular pH of 7.4 after SV exocytosis and is almost maximal at the intravesicular pH of 5.5 (Hua et al., 2011). Therefore, the cup-shaped structures observed in Figure 1A can be assumed to be calyx terminals filled with anti-Syt2-cypHer-containing internalized vesicles. WebBODIPY dye conjugates of lipids, toxins, steroids, drug analogs and receptor probes typically have quantum yields approaching unity, especially in organic solvents. The low … ipcc 4th assessment
Cytiva CypHer5E NHS Esters:Protein Analysis …
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